Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes. In doing so you will learn how HPLC methods work even if you never need to develop a new method. This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes.
Additionally, learn how to implement strategies to achieve satisfactory separation for ‘complex’ samples and to find solutions for difficult HPLC separations in order to develop robust and fit for purpose HPLC methods.
This course focuses primarily on on strategies for reversed phase HPLC separations.
Attendees are invited to bring along any real life examples that they would like advice on during the training. These may be discussed during group exercises, or, where intellectual property is an issue, privately with the trainer.
Total learning time = 10 hours
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