How to Run HPLC Methods

This course is a great starting point for those wanting to use HPLC. It will introduce all of the method parameters and explain how you go from that to settting up and interpreting a chromatographic analysis.


Learn how to set up and run HPLC analysis with full understanding of all the method parameters such as the column, the mobile phase, the instrumentation, and sample preparation, and how to interpret the results of the analysis.

The course is delivered using a combination of presentations and exercises in which extensive use of case studies provides real life experience. It will enable you to implement HPLC analytical methods by transferring the parameters from the method to your HPLC system.

Attendees are invited to bring along any real life examples that they would like advice on during the training. These may be discussed during group exercises, or, where intellectual property is an issue, privately with the trainer.

Total learning time = 5 hours

More about total learning time

Learning Objectives

On completion of this training course you will be able to:

  1. Understand what is meant by all the parameters in an HPLC analytical method.
  2. Follow an HPLC analytical method to set up an HPLC system for analysis.
  3. Run an HPLC analytical method and acquire chromatographic results.
  4. Interpret chromatograms obtained from HPLC analysis.
How is this course delivered?

This course is delivered as a 1 day ‘virtual’ live online training event which is delivered over a 6 hour period, including breaks, typically from 9am to 3pm (but may depend on your time zone).

More about live online training

Total learning time = 5 hours

More about total learning time

The full agenda is provided in the course description.

The course may also be delivered as in-house training, either in the live online format or in a classroom based format at your site. In-house training allows customisation to meet your specific requirements.

More about in-house training

Who will deliver this training course?

All training courses are delivered by the MTS consultant, Oona McPolin. Some recordings of MTS webinars are provided below as examples of Oona’s presentation style.

Strategies for HPLC Method Development
Webinar Recording This webinar explored the different strategies, and combinations of strategies, which may be applied during HPLC method development. …
Where do the Acceptance Criteria in Method Validation Come From?
Webinar Recording One of the most difficult tasks when writing an analytical method validation protocol is to set suitable acceptance …
Who should attend this course?

Those who are new to HPLC and want to know more.

For example:

  • Analytical chemists in industry (the course is not specific to a particular industry)
  • Researchers who need to use HPLC
When can I attend this course in 2024?
  • Thu 18th Apr 2024 (9am to 3pm BST/UTC+1)
  • Tue 17th Sep 2024 (9am to 3pm BST/UTC+1)
How much does it cost?

£275 GBP or €315 EUR

Included in your course fees:
  • Comprehensive course hand-outs – The training book is provided as an electronic copy (pdf) for both live online and classroom based options.
  • Certificate of Attendance
  • Optional post training assessment (accessed in e-MTS, our learning management system) which leads to a Certificate of Training.
  • Access to training materials via e-MTS
  • Post training support – Attendees can contact the trainer with questions that may occur when they apply their learning to real life situations.
  1. All prices are quoted per person excluding VAT.
  2. VAT notice for live online training: For UK business cutomers and non-business customers (B2C) from all countries VAT is charged at the UK rate (currently 20%). For EU business customers (B2B), the reverse charge can be applied on receipt of a valid VAT identification number and VAT will not be charged. For business customers outside of the UK and EU, VAT will not be charged.
Cancellations and Substitutions

Once made and accepted by MTS, course bookings are liable for payment. Payment is due at least 14 days prior to the course start date. Cancellations prior to 3 weeks before the course date are subject to an administration charge of 25%. Cancellations received less than 3 weeks before the start of the course will not be refunded, although substitutions may be made at any time.

We reserve the right to change the programme, speakers, date or venue without notice, or to cancel an event. In the unlikely event of cancellation occurring, delegates will be notified as soon as possible and will receive a full refund of fees paid.

Related Courses:
HPLC Mobile Phase Expiry Dates
Question: What is the typical (the industrial 'norm') for the expiration date of mobile phases …
Replicates in Analytical Methods: When and How Many?
Do you prepare your samples/standards in duplicate without really thinking about it? If challenged on …
HPLC Autosampler Vials: Dos and Don’ts
The HPLC vial is a seemingly minor component of an HPLC system but it has …