Our course, ‘How to Troubleshoot HPLC’ is now available as a self-paced online training course. This means you can access the course over a period of 1 month in our virtual learning environment (e-MTS) in the form of on demand videos and exercises that you complete at a time to suit you. It’s perfect if […]
Tag: HPLC
How Long to Equlibrate an HPLC Column?
MTS HELPDESK: How long does it take to equilibrate a column on an HPLC system before the first injection can be performed?
HPLC Mobile Phase Expiry Dates
What is the typical (the industrial ‘norm’) for the expiration date of mobile phases for HPLC, i.e. with buffer and without buffer. Also, what should you do (e.g., measure the pH or run and verify the retention time) if you need to extend the expiration date in extenuating circumstance e.g. shortage of acetonitrile or delay of the orders from the vendor, etc.?
Replicates in Analytical Methods: When and How Many?
The use of replicates in analytical procedures is commonplace but it is important to understand why you are doing it and also what the results are telling you.
HPLC Autosampler Vials: Dos and Don’ts
The HPLC vial is a seemingly minor component of an HPLC system but it has the potential to create major problems. This is particularly true for quantitative analysis. The following list of dos and don’ts will help you to avoid some of the more common pitfalls.
Do I Have to Revalidate a Method if the HPLC Column is Changed?
I would like to have a ‘back-up’ column for my HPLC method so that I am not reliant on one supplier, but how can I choose one that will be suitable and do I need to validate the method fully on the replacement column?