Our consultant will answer your questions and provide advice on your technical problems. Use our enquiry form to send us your questions.

Questions and answers may end up as posts on our blog. You can use browse some previously submitted questions below:

• What is an Analytical Procedure Lifecycle?
• Limit of Quantitation: Does it Matter?
• How Long to Equlibrate an HPLC Column?
• HPLC Mobile Phase Expiry Dates
• Replicates in Analytical Methods: When and How Many?
• How Much Forced Degradation in a Stress Study?
• HPLC Autosampler Vials: Dos and Don’ts
• Do I Have to Revalidate a Method if the HPLC Column is Changed?
• What is Mass Balance in a Forced Degradation Study?
• Why are ‘Bio’ Test Methods so Tricky to Validate?
• Where do the Acceptance Criteria in Method Validation Come From?

The MTS HPLC calculator is in the form of an Excel spreadsheet comprised of 7 worksheets which correspond to useful HPLC related calculations.

The calculator was developed to support our HPLC training courses, ‘How to Run HPLC Methods‘, ‘How to Troubleshoot HPLC‘ and ‘How to Develop HPLC Methods‘.

It has been downloaded hundreds of times by users from all over the world. If you have any requests or feedback then please let us know.

Simply fill in and submit the Request Form below (scroll down to the bottom of this webpage), your free HPLC calculator will be sent to you by email (so you may need to check your junk folder).

The calculations provided are as follows:

#1 Column Equilibration

For how long do you need to equilibrate a column before it is ready to use?

#2 Void Volume

Calculates the void volume and estimates the retention time of t0 (often referred to as the solvent front).

#3 Pressure Converter

Change pressure units between the most commonly used units, e.g. psi to bar

#4 Mobile Phase Buffer Strength

Performs the conversion from millimoles (mM) and %w/w to a mass and volume.

#5 Mobile Phase Quantity – Isocratic

Works out how much mobile phase you will need to prepare for an isocratic analysis.

#6 Mobile Phase Quantity – Gradient

Works out how much mobile phase you will need to prepare for a gradient analysis (one of the most popular!).

#7 Method Development

Accompanies our HPLC method development courses to provide a simple way to convert a single gradient scouting run into a prediction of best conditions for initial method development, and predict whether an isocratic or a gradient approach will be most suitable. 

On our blog you will find articles and videos on topics related to chemical analysis, mostly in a pharma/biopharma context. Recent posts include:

Forced Degradation (1) HPLC (8) ICH (10) Lifecycle (5) Method development (2) Methods (1) MTS (2) MTS Helpdesk (11) OOS/OOT (1) Procedures (1) Stability (3) Troubleshooting (1) Validation (15) Verification (1) Video (5) Webinar (2)

On-demand webinar recordings:

Some recordings of MTS webinars are provided below. These are intended as useful resources but also provide a sample of the presentation style of the MTS consultant, Oona McPolin, who delivers all of our training courses.

The purpose of this aide mémoire is to provide a list of method features that may be important to consider during a transfer. It provides a convenient means to identify any potential issues and record the findings during a risk assessment gap analysis.

The tool was developed to support our training course on method verification and transfer. If you have any suggestions to improve the tool, then please let us know.

Simply fill in and submit the Request Form below, your free aide mémoire will be sent to you by email (so you may need to check your junk folder).